Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening

Tumor organoids are promising tools for cancer biology investigations and pre-clinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings.