Pharmacogenomics of thiopurines: distribution of TPMTand NUDT15polymorphisms in the Brazilian Amazon

Supplemental Digital Content is available in the text.Reduced function alleles in the TPMTand NUDT15genes are risk factors for thiopurine toxicity. This study evaluated the influence of Native ancestry on the distribution of TPMT(rs1142345, rs1800460 and rs1800462) and NUDT15(rs116855232) polymorphisms and compound metabolic phenotypes in 128 healthy males from the Brazilian Amazon. The average proportion of Native and European ancestry differed greatly and significantly between self-declared Amerindians and non-Amerindians, although extensive admixture in both groups was evident. Native ancestry was not significantly associated with the frequency distribution of the TPMTor NUDT15polymorphisms investigated. The apparent discrepancy with our previous results for NUDT15rs116855232 in the Ad Mixed American superpopulation of the 1000 Genomes Project is ascribed to the diversity of the Native populations of the Americas. Based on the inferred TPMT/NUDT15 compound metabolic phenotypes, the Clinical Pharmacogenetics Implementation Consortium recommendations for starting thiopurine therapy with reduced doses or to consider dose reduction applied respectively to 3–5% and to 12–20% of the study cohorts.