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Rnf20deficiency in adipocyte impairs adipose tissue development and thermogenesis

Authors :
Liang, Xiaojuan
Tao, Cong
Pan, Jianfei
Zhang, Lilan
Liu, Lulu
Zhao, Ying
Fan, Yiping
Cao, Chunwei
Liu, Jiali
Zhang, Jin
Lam, Sin Man
Shui, Guanghou
Jin, Wanzhu
Li, Wei
Zhao, Jianguo
Li, Kui
Wang, Yanfang
Source :
Protein & Cell; June 2021, Vol. 12 Issue: 6 p475-492, 18p
Publication Year :
2021

Abstract

RNF20, an E3 ligase critical for monoubiquitination of histone H2B at lysine 120 (H2Bub), has been implicated in the regulation of various cellar processes; however, its physiological roles in adipocytes remain poorly characterized. Here, we report that the adipocyte-specific knockout of Rnf20(ASKO) in mice led to progressive fat loss, organomegaly and hyperinsulinemia. Despite signs of hyperinsulinemia, normal insulin sensitivity and improved glucose tolerance were observed in the young and aged CD-fed ASKO mice. In addition, high-fat diet-fed ASKO mice developed severe liver steatosis. Moreover, we observed that the ASKO mice were extremely sensitive to a cold environment due to decreased expression levels of brown adipose tissue (BAT) selective genes, including uncoupling protein 1 (Ucp1), and impaired mitochondrial functions. Significantly decreased levels of peroxisome proliferator-activated receptor gamma (PparĪ³) were observed in the gonadal white adipose tissues (gWAT) from the ASKO mice, suggesting that Rnf20regulates adipogenesis, at least in part, through PparĪ³. Rosiglitazone-treated ASKO mice exhibited increased fat mass compared to that of the non-treated ASKO mice. Collectively, our results illustrate the critical role of RNF20 in control of white and brown adipose tissue development and physiological function.

Details

Language :
English
ISSN :
1674800X and 16748018
Volume :
12
Issue :
6
Database :
Supplemental Index
Journal :
Protein & Cell
Publication Type :
Periodical
Accession number :
ejs53975340
Full Text :
https://doi.org/10.1007/s13238-020-00770-2