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Quantification of prostaglandins E2and D2using liquid chromatography-tandem mass spectrometry in a mouse ear edema model
- Source :
- Analytical Methods; 2020, Vol. 12 Issue: 22 p2865-2871, 7p
- Publication Year :
- 2020
-
Abstract
- A sensitive, specific, and accurate high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the quantification of prostaglandins D2(PGD2) and E2(PGE2) in a mouse ear edema model. We used activated charcoal to obtain PG-free ear samples. The chromatographic separation was performed using a Hypersil Gold C18 column. The limit of detection of each PG was 0.4 ng mL−1, and the intra- and inter-assay estimates of precision and accuracy were <14.5 and 94.2–102.9%, respectively. Stability studies showed that all analytes were stable under various storage conditions and analytical processes. The developed and validated method was successfully used to investigate the anti-inflammatory effects of cultured bear bile powder (CBBP) by quantitatively determining PGE2and PGD2levels in mouse ear edema samples. These results showed that CBBP significantly inhibited the xylene-induced ear edema in mice and reversed the xylene-induced elevation of PGE2and PGD2levels. These results provide useful data about the anti-inflammatory bioactivities in tissues, mediated by the reduction of PGE2and PGD2levels, and may further encourage research and development studies of CBBP for its use as an anti-inflammatory agent.
Details
- Language :
- English
- ISSN :
- 17599660 and 17599679
- Volume :
- 12
- Issue :
- 22
- Database :
- Supplemental Index
- Journal :
- Analytical Methods
- Publication Type :
- Periodical
- Accession number :
- ejs53471311
- Full Text :
- https://doi.org/10.1039/d0ay00506a