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Intelligent image-based deformation-assisted cell sorting with molecular specificity
- Source :
- Nature Methods; June 2020, Vol. 17 Issue: 6 p595-599, 5p
- Publication Year :
- 2020
-
Abstract
- Although label-free cell sorting is desirable for providing pristine cells for further analysis or use, current approaches lack molecular specificity and speed. Here, we combine real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves and transfer molecular specificity to image-based sorting using an efficient deep neural network. In addition to general performance, we demonstrate the utility of this method by sorting neutrophils from whole blood without labels.
Details
- Language :
- English
- ISSN :
- 15487091 and 15487105
- Volume :
- 17
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Nature Methods
- Publication Type :
- Periodical
- Accession number :
- ejs53317539
- Full Text :
- https://doi.org/10.1038/s41592-020-0831-y