Inhibitory Effects of Indole-3-Acetonitrile-4-Methoxy-2-S-Β-d-Glucopyranoside From Nasturtium officinaleR. Br on Human Kallikrein 5 and 7 Protease

The stratum corneum tryptic enzyme kallikrein 5 (KLK5) is a serine protease that is involved in the cell renewal and maintenance of the skin barrier functions. The excessive activation of KLK5 causes an exacerbation of dermatoses, such as rosacea and atopic dermatitis. Nasturtium officinaleR. Br. (Brassicaceae) is a hardy perennial plant native to Europe and commonly called “Watercress” or “Cresson,” which is one of the brassica vegetables. We aimed to investigate whether the bioactive sulfur-containing compound inhibits the KLK5 protease. The sulfur-containing compounds were evaluated using an enzymatic assay to measure the anti-KLK5 activity. Our study revealed that the sulfur-containing compound inhibits the KLK5 and 7 protease activity. Indole-3-acetonitrile-4-methoxy-2-S-β-d-glucopyranoside may affect the skin barrier and atopic function via the regulation of proteases.