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Development and identification of glyphosate-tolerant transgenic soybean viadirect selection with glyphosate

Authors :
GUO, Bing-fu
HONG, Hui-long
HAN, Jia-nan
ZHANG, Li-juan
LIU, Zhang-xiong
GUO, Yong
QIU, Li-juan
Source :
Journal of Integrative Agriculture; May 2020, Vol. 19 Issue: 5 p1186-1196, 11p
Publication Year :
2020

Abstract

Glyphosate-tolerant soybean is the most widely planted genetically modified crop worldwide. However, soybean remains recalcitrant to routine transformation because of the low infection efficiency of Agrobacteriumto soybean and lack of useful selectable markers. In this study, several Agrobacteriumstrains and cell densities were compared by transient expression of the GUSgene. The results showed that Agrobacteriumstrain Ag10 at cell densities of OD600of 0.6–0.9 yielded the highest infection efficiency in Agrobacterium-mediated soybean cotyledonary node transformation system. Meanwhile, a simple and rapid method was developed for identification of glyphosate tolerance in putative T0transgenic plants, consisting of spotting plantlets with 1 µL Roundup®. The whole cycle of genetic transformation could be shortened to about 3 mon by highly efficient selection with glyphosate during the transformation process and application of the spot assay in putative T0transgenic plantlets. The transformation frequency ranged from 2.9 to 5.6%. This study provides an improved protocol for development and identification of glyphosate-tolerant transgenic soybeans.

Details

Language :
English
ISSN :
20953119
Volume :
19
Issue :
5
Database :
Supplemental Index
Journal :
Journal of Integrative Agriculture
Publication Type :
Periodical
Accession number :
ejs52815612
Full Text :
https://doi.org/10.1016/S2095-3119(19)62747-4