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A mathematical model of the regulation of the G1phase of Rb +/+ and Rb —/— mouse embryonic fibroblasts and an osteosarcoma cell line

Authors :
Obeyesekere, M. N.
Knudsen, E. S.
Wang, J. Y. J.
Zimmerman, S. O.
Source :
Cell Proliferation; March 1997, Vol. 30 Issue: 3-4 p171-194, 24p
Publication Year :
1997

Abstract

A mathematical model integrating the roles of cyclin D, cdk4, cyclin E, cdk2, E2F and RB in control of the G1phase of the cell cycle is described. Experimental results described with murine embryo fibroblasts (MEFs), either Rb +/+ or Rb —/—, and with the RB‐deficient osteosarcoma cell line, Saos‐2, served as the basis for the formulation of this mathematical model. A model employing the known interactions of these six proteins does not reproduce the experimental observations described in the MEFs. The appropriate modelling of G1requires the inclusion of a sensing mechanism which adjusts the activity of cyclin E/cdk2 in response to both RB concentration and growth factors. Incorporation of this sensing mechanism into the model allows it to reproduce most of the experimental results observed in Saos‐2 cells, Rb —/— MEFS, and Rb +/+ MEFs. The model also makes specific predictions which have not been tested experimentally.

Details

Language :
English
ISSN :
09607722 and 13652184
Volume :
30
Issue :
3-4
Database :
Supplemental Index
Journal :
Cell Proliferation
Publication Type :
Periodical
Accession number :
ejs52721844
Full Text :
https://doi.org/10.1111/j.1365-2184.1997.tb00933.x