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Gene gymnastics

Authors :
Vijayachandran, Lakshmi S
Thimiri Govinda Raj, Deepak B
Edelweiss, Evelina
Gupta, Kapil
Maier, Josef
Gordeliy, Valentin
Fitzgerald, Daniel J
Berger, Imre
Source :
Bioengineered; September 2013, Vol. 4 Issue: 5 p279-287, 9p
Publication Year :
2013

Abstract

Most essential activities in eukaryotic cells are catalyzed by large multiprotein assemblies containing up to ten or more interlocking subunits. The vast majority of these protein complexes are not easily accessible for high resolution studies aimed at unlocking their mechanisms, due to their low cellular abundance and high heterogeneity. Recombinant overproduction can resolve this bottleneck and baculovirus expression vector systems (BEVS) have emerged as particularly powerful tools for the provision of eukaryotic multiprotein complexes in high quality and quantity. Recently, synthetic biology approaches have begun to make their mark in improving existing BEVS reagents by de novo design of streamlined transfer plasmids and by engineering the baculovirus genome. Here we present OmniBac, comprising new custom designed reagents that further facilitate the integration of heterologous genes into the baculovirus genome for multiprotein expression. Based on comparative genome analysis and data mining, we herein present a blueprint to custom design and engineer the entire baculovirus genome for optimized production properties using a bottom-up synthetic biology approach.

Details

Language :
English
ISSN :
21655979 and 21655987
Volume :
4
Issue :
5
Database :
Supplemental Index
Journal :
Bioengineered
Publication Type :
Periodical
Accession number :
ejs52436358
Full Text :
https://doi.org/10.4161/bioe.22966