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Increased migration of a human glioma cell line after in vitro CyberKnife irradiation
- Source :
- Cancer Biology and Therapy; October 2011, Vol. 12 Issue: 7 p629-633, 5p
- Publication Year :
- 2011
-
Abstract
- A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8Gy was delivered intermittently over a period of 40 minutes. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF-B at four days (Real time PCR), β1-integrin at 4-5 days (real-time PCR and western blot) and no elevation in phosphorylated AKT at days 4 and 5 (western blot). Our data suggests that glioma cell invasion as well as elevations of TGF-B and β1-integrin are associated with IIR and not CIR.
Details
- Language :
- English
- ISSN :
- 15384047 and 15558576
- Volume :
- 12
- Issue :
- 7
- Database :
- Supplemental Index
- Journal :
- Cancer Biology and Therapy
- Publication Type :
- Periodical
- Accession number :
- ejs52436107
- Full Text :
- https://doi.org/10.4161/cbt.12.7.16862