In-vitro evaluation of antimicrobial, antioxidant, alpha-amylase inhibition and cytotoxicity properties of Cannabis sativa

Cannabis sativa(C. sativa) is a widespread medicinal plant used for humans and cattles infectious diseases. The current study is carried out to assess phytochemical, antimicrobial, antioxidant, α-amylase inhibition and cytotoxic activity of the Cannabis sativaplant. An appropriate amount of phytochemicals like tannins, flavonoids, lycopene, and β-carotenes is found in C. sativaplant during the phytochemical screening. Among these phytochemicals of C. sativaplant, tannin shows outstanding activity. The plant extracts are assessed for antimicrobial activity against different seven bacterial strains. Notably, the stem part of C. sativais found to exhibit higher antimicrobial potential than root and leaf. Among the extracts, the antioxidant assays (evaluated through % inhibition of linoleic acid peroxidation assessment) of n-hexane extract of leaf part was highest (80.70%) compared to the others. The plant also contains a potent α-amylase inhibitory potential, the root extract shows maximum inhibition of α-amylase. The cytotoxic effect [evaluated through hemolytic activity as (%) lysis of RBCs against human erythrocytes] of the plant extracts is found insignificant ranging from 1.97 to 5.88. The plant C. sativakeeps a significant amount of bioactive phenolic combinations accompanied by high antimicrobial and antioxidant activities.