Back to Search
Start Over
Citreoviridin, a specific inhibitor of the mitochondiral adenosine triphosphatase
- Source :
- Biochemical Journal; March 1978, Vol. 170 Issue: 3 p503-510, 8p
- Publication Year :
- 1978
-
Abstract
- 1. Citreoviridin was a potent inhibitor of the soluble mitochondrial ATPase (adenosine triphosphatase) similar to the closely related aurovertins B and D. 2. Citreoviridin inhibited the following mitochondrial energy-linked reactions also: ADP-stimulated respiration in whole mitochondria from ox heart and rat liver; ATP-driven reduction of NAD+ by succinate; ATP-driven NAD transhydrogenase and ATPase from ox heart submitochondrial particles. 3. The dissociation constant (KD) calculated by a simple law-of-mass-action treatment for the citreoviridin–ATPase complex was 0.5–4.2micron for ox-heart mitochondrial preparations and 0.15micron for rat liver mitochondria. 4. Monoacetylation of citreoviridin decreased its inhibitory potency (KD=2–25micron, ox heart; KD=0.7micron, rat liver). Diacetylation greatly decreased the inhibitory potency (KD=60–215micron, ox heart). 5. Hydrogenation of citreoviridin monoacetate diminished its inhibitory potency considerably. 6. No significant enhancement of fluorescence was observed when citreoviridin interacted with the mitochondrial ATPase.
Details
- Language :
- English
- ISSN :
- 02646021 and 14708728
- Volume :
- 170
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Biochemical Journal
- Publication Type :
- Periodical
- Accession number :
- ejs51291429
- Full Text :
- https://doi.org/10.1042/bj1700503