Expression of gp145 trkB in the early stage of Schwann cell tube formation

Abstract: : We investigated the expression of tyrosine kinase receptors (trkBs) and their ligand, brain-derived neurotrophic factor (BDNF), in isolated sciatic nerve segments of rat in a silicone chamber model devoid of axonal contact, using an immunohistochemical technique. The receptor gp 95 ^trkB was not detected during the first 3 weeks but was strongly expressed in the nerve stumps on day 21. In contrast, gp 145 ^trkB immunoreactivity was readily detected in the Schwann cells in the nerve stumps between days 1 and 7 after isolation. Immunohistochemical analysis using anti-S-100 antibody demonstrated that Schwann cell tube formation within the silicone chamber had been completed by the third week, which suggested that gp 95 ^trkB began to be produced by Schwann cells after completion of Schwann cell tube formation. Considering the low level of BDNF production during the first week, molecules other than BDNF may be ligands for gp 145 ^trkB in the silicone chamber model devoid of axonal contact.