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Technical Note: Detection of cat, dog, and rat or mouse tissues in food and animal feed using species-specific polymerase chain reaction1
- Source :
- Journal of Animal Science; October 2007, Vol. 85 Issue: 10 p2734-2739, 6p
- Publication Year :
- 2007
-
Abstract
- A PCR method based on the nucleotide sequence variation in the 12S ribosomal RNA, mitochondrial gene has been developed for the specific and qualitative detection and identification of cat, dog, and rat or mouse tissue in food and feedstuffs. The primers designed generated specific fragments of 108, 101, and 96 bp in length for cat, dog, and rat or mouse tissues, respectively. Specificity of the primers was tested against 32 nontarget species including mammals, birds, fish, and plant species. This PCR method allowed detection of raw and heated cat, dog, and rat or mouse tissues in meat/oats mixtures even when the concentration of the target species was reduced to 0.1%. Furthermore, the performance of the method was not affected by prolonged heat-treatment (up to 133°C for 20 min at 300 kPa), and consequently, it could be very useful to verify the origin of raw materials in food and feedstuffs submitted to denaturing technologies, for which other methods cannot be applied.
Details
- Language :
- English
- ISSN :
- 00218812 and 15253163
- Volume :
- 85
- Issue :
- 10
- Database :
- Supplemental Index
- Journal :
- Journal of Animal Science
- Publication Type :
- Periodical
- Accession number :
- ejs50386379
- Full Text :
- https://doi.org/10.2527/jas.2007-0048