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Single-Cell and Time-Resolved Profiling of Intracellular SalmonellaMetabolism in Primary Human Cells
- Source :
- Analytical Chemistry; June 2019, Vol. 91 Issue: 12 p7729-7737, 9p
- Publication Year :
- 2019
-
Abstract
- The intracellular pathogen Salmonella entericahas evolved an array of traits for propagation and invasion of the intestinal layers. It remains largely elusive how Salmonellaadjusts its metabolic states to survive inside immune host cells. In this study, single-cell Raman biotechnology combined with deuterium isotope probing (Raman-DIP) have been applied to reveal metabolic changes of the typhoidal SalmonellaTyphi Ty2, the nontyphoidal SalmonellaTyphimurium LT2, and a clinical isolate Typhimurium D23580. By initially labeling the Salmonellastrains with deuterium, we employed reverse labeling to track their metabolic changes in the time-course infection of THP-1 cell line, human monocyte-derived dendritic cells (MoDCs) and macrophages (Mf). We found that, in comparison with a noninvasive serovar, the invasive Salmonellastrains Ty2 and D23580 have downregulated metabolic activity inside human macrophages and dendritic cells and used lipids as alternative carbon source, perhaps a strategy to escape from the host immune response. Proteomic analysis using high sensitivity mass spectrometry validated the findings of Raman-DIP analysis.
Details
- Language :
- English
- ISSN :
- 00032700 and 15206882
- Volume :
- 91
- Issue :
- 12
- Database :
- Supplemental Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs50220630
- Full Text :
- https://doi.org/10.1021/acs.analchem.9b01010