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EVIDENCE FOR DOUBLE INFECTION WITHIN SOYBEAN NODULES

EVIDENCE FOR DOUBLE INFECTION WITHIN SOYBEAN NODULES

Authors :
LINDEMANN, W. C.
SCHMIDT, E. L.
HAM, G. E.
Source :
Soil Science; October 1974, Vol. 118 Issue: 4 p274-279, 6p
Publication Year :
1974

Abstract

Rhizobium japonicumstrains 117 and 138 (USDA) were used in equal proportions to inoculate soybean seedlings. Preparations of individual nodules were stained with strain-specific fluorescent antibodies and rhizobia present were identified by direct microscopic examination using transmitted tungsten (darkfield) light and reflected near-ultraviolet light singly and in combination. At an inoculation density of 108rhizobia per plant, 32 percent of the nodules contained both strains. The percent double infection decreased as inoculum density decreased. Strain 138 generally predominated over strain 117 in accounting for the majority of the nodules that contained only one strain, and cells of 138 were generally numerically predominant in nodules that contained both strains. Streak plate cultures of selected nodule slurries provided data that fully reinforced the fluorescent-antibody data.

Details

Language :
English
ISSN :
0038075X and 15389243
Volume :
118
Issue :
4
Database :
Supplemental Index
Journal :
Soil Science
Publication Type :
Periodical
Accession number :
ejs49322959