Characterization of a cDNA clone encoding pedal peptide in the terrestrial snail

WE report the isolation of a Helix lucorumcDNA clone encoding a precursor of neuropeptides that are closely related to Aplysiaand Tritoniapedal peptides (Pep). The predicted propeptide contains 20 copies of the two variants of HelixPep interspersed with Lys-Arg endopeptidase cleavage sites. Northern blot hybridization revealed multiple Pep-hybridizing species in the HelixCNS RNA. The Pep gene was expressed by several identified serotonergic neurones in pedal and cerebral ganglia, groups of sensory neurones in procerebrum, peripheral neurones in olfactory bulb, mantle and foot, and group of neurones in pedal ganglia presumably involved in locomotion control. Pep mRNA was detected in several neurones at the early stages of nervous system development.