PP.28.12

Components of the Renin-Angiotensin-Aldosterone-System (RAAS) are expressed and regulated in vascular endothelial cells. In particular, the epithelial Na-channel (ENaC), the mineralocorticoid receptor (MR), and the NaK-ATPase have been reported to regulate endothelial stiffness in response to serum Na. In the current work, we determined the effect of sAC inhibition on endothelial stiffness. Furthermore, we analyzed sAC-dependent CRE-mediated transcriptional activation and the effects of sAC inhibition on ENaC-, NaK-ATPase-- and MR expression on mRNA and protein levels.