Phlebiopsis yunnanensissp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analysis

A new wood-inhabiting fungal species,Phlebiopsis yunnanensissp. nov., is proposed based on morphological and molecular characters. The species is characterized by resupinate, membranaceous to subceraceous basidiocarps, a monomitic hyphal system with simple separated generative hyphae, cystidia conical, thick-walled, heavily encrusted with large crystals in the apical part and ellipsoid basidiospores measuring 3.5–4.5×2.5–3.5 μm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed thatP. yunnanensisbelonged to the Phanerochaetaceae and nested into the phlebioid clade. Further investigation was obtained for more representative taxa in thePhlebiopsisbased on ITS+nLSU sequences, in which the result demonstrated that the speciesP. yunnanensisformed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and then grouped withP. gigantea andP. lamprocystidiata.