The jasmonate‐induced 60 kDa protein of barley exhibits N‐glycosidase activity in vivo

Upon jasmonate treatment barley leaf segments express a putative ribosome‐inactivating protein (JIP60). The influence of this protein on translation in planta has been analysed by using barley plants and tobacco plants transformed with a barley cDNA encoding JIP60. In both plant systems JIP60 exhibited N‐glycosidase activity in vivo. The depurination of the 25S rRNA of tobacco and barley ribosomes led to accumulation of translationally inactive polysomes.