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Telomerase from Saccharomyces cerevisiaecontains several protein subunits and may have different activities depending on the protein content
- Source :
- FEBS Letters; September 1998, Vol. 436 Issue: 1 p35-40, 6p
- Publication Year :
- 1998
-
Abstract
- Telomerase is a ribonucleoprotein responsible for maintaining telomeres during the cell cycle [1, 2]. Here we describe a two‐step purification procedure for the Saccharomyces cerevisiaetelomerase complex. We have found that the properties (processivity, nuclease activity) of telomerase depend on the isolation procedure. Using a cross‐linking approach, we have revealed several proteins that could be components of the telomerase complex. Furthermore, spectra of cross‐linked proteins differ in processive and non‐processive telomerase complexes.
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 436
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- FEBS Letters
- Publication Type :
- Periodical
- Accession number :
- ejs46679862
- Full Text :
- https://doi.org/10.1016/S0014-5793(98)01091-6