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Formation of benzo(α)pyrene metabolites and DNA adducts catalyzed by a rat liver mitochondrial monooxygenase system
- Source :
- Biochemical and Biophysical Research Communications; September 1985, Vol. 131 Issue: 2 p935-942, 8p
- Publication Year :
- 1985
-
Abstract
- Sonic disrupted mitoplasts from 3-methylcholanthrene (MCA) treated rats can catalize the formation of benzo(a)pyrene (BaP) adducts with calf thymus DNA in the presence of an NADPH generating system. The mitoplasts used in this study contained<1% microsomal marker enzymes: rotenone insensitive NADPH cytochrome c reductase and glucose-6-phosphatase. The rates of BaP metabolism and DNA adduct formation per nanomole cytochrome P-450 were different for MCA induced mitochondrial and microsomal enzymes. The major B(a)P DNA adducts formed in incubations with lysed mitoplasts were derived from reaction of 9-OH-B(a)P-4,5 oxide with deoxyguanosine. The results suggest a potential role of mitochondrial monooxygenase activity in the covalent binding of B(a)P to mitochondrial DNA.
Details
- Language :
- English
- ISSN :
- 0006291X and 10902104
- Volume :
- 131
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Biochemical and Biophysical Research Communications
- Publication Type :
- Periodical
- Accession number :
- ejs45632923
- Full Text :
- https://doi.org/10.1016/0006-291X(85)91329-4