Microbiological transformation 32: Use of epoxide hydrolase mediated biohydrolysis as a way to enantiopure epoxides and vicinal diols: Application to substituted styrene oxide derivatives

The biohydrolyses of various substituted styrene oxide derivatives using the fungi Aspergillus nigeror Beauveria sulfurescensare described. The results obtained show that this methodology allows the preparation of enantiomerically enriched epoxides and diols viaenantioselective and regioselective hydration. The comparative study of the results obtained suggests that these hydrolyses operate following different mechanisms and a model of the corresponding active sites is proposed.