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Effects of Dialysis Membranes on the Kinetics of Tumor Necrosis Factor-α Production by Peripheral Mononuclear Cells in Chronic Hemodialysis Patients
- Source :
- International Journal of Artificial Organs; July 1998, Vol. 21 Issue: 7 p384-390, 7p
- Publication Year :
- 1998
-
Abstract
- To evaluate the biocompatibility of dialysis membranes, blood samples were collected from 10 hemodialysis patients immediately before dialysis and peripheral blood mononuclear cells were isolated. The 3.0 x 105cells/ml were then passed 30 times through modules made of a polyethylene glycolgrafted cellulose membrane, a polyacrylonitrile membrane, and a polysulfone membrane. Expression of messenger RNA for tumor necrosi factor-α (TNF-α) was determined. Cells were also cultured for 2 h with and without lipopolysaccharide and TNF-α levels in the supernatant were measured.TNF-α messenger RNA expression was significantly higher immediately after passage through the polyacrylonitrile membrane compared with the other membranes. Cells cultured without lipopolysaccharide, produced significantly less TNF-α after passage through the polysulfone membrane, while lipopolysaccharide significantly increased TNF-α production by cells passed through the polyacrylonitrile membrane. These results suggest that biocompatibility differs even among dialysis membranes believed to cause no complement activation.
Details
- Language :
- English
- ISSN :
- 03913988 and 17246040
- Volume :
- 21
- Issue :
- 7
- Database :
- Supplemental Index
- Journal :
- International Journal of Artificial Organs
- Publication Type :
- Periodical
- Accession number :
- ejs45029062
- Full Text :
- https://doi.org/10.1177/039139889802100706