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Neat Protein–Polymer Surfactant Bioconjugates as Universal Solvents
- Source :
- Biomacromolecules; 20240101, Issue: Preprints
- Publication Year :
- 2024
-
Abstract
- Solvents, particularly those having low volatility, are of great interest for the biocatalytic synthesis of utility chemicals and fuels. We show novel and universal solvent-like properties of a neat and water-less polymer surfactant–bovine serum albumin (BSA) conjugated material (WL-PSpBSA). This highly viscous, nonvolatile material behaves as a liquid above its solid–liquid transition temperature (∼25–27 °C) and can be used as a solvent for variety of completely dry solutes of different sizes and surface chemistries. We show using a combination of optical microscopy and steady -state and time-resolved fluorescence spectroscopy that dry and powdered dyes (hydrophobic Coumarin 153 (C153)), enzymes (α-chymotrypsin (α-Chy)), or even 1 μm microparticles (diffusion coefficient ca. three orders slower than C153), can be solubilized and completely dispersed in the WL-PSpBSA solvent above 25 °C. While C153, irrespective of its mode of addition to WL-PSpBSA, binds similarly to BSA, α-Chy can be stabilized and activated to perform its hydrolysis function, even at 100 °C. This work therefore provides insights into the form of universal solvent characteristic property for this new class of nonaqueous, nonvolatile, biodegradable protein–polymer surfactant-based conjugated materials and suggests potential new avenues that can have a huge impact on biocatalysis, bionanotechnology, drug delivery, and other applications.
Details
- Language :
- English
- ISSN :
- 15257797 and 15264602
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Biomacromolecules
- Publication Type :
- Periodical
- Accession number :
- ejs44833033
- Full Text :
- https://doi.org/10.1021/acs.biomac.7b01729