Phytofabrication, characterisation and antimicrobial studies of gold nanoparticles using Cannabis sativa (Bhang), leaf extract

Phytofabrication of gold nanoparticles by plant extract of cannabis sativa is at present under exploitation for the reason to develop environmentally benign nanoparticles synthesis to avoid adverse effects in biomedical applications. Aqueous solution of HAuCl44H2O was taken to react with different concentration of ethanolic leaf extract of the plant. A fixed ratio of plant extract and metal ion was mixed which in turn showed the change in colour of the solution leading to the inference that nanoparticles are formed. The characterisation of nanoparticles was done by UV-VIS Spectrophotometer, FTIR, and TEM. The particles synthesised were of the size ranging from 20-100 nm. The solution showed a peak at 560 nm on UV-VIS spectrum corresponding to the surface Plasmon resonance of gold nanoparticles. The shape of the nanoparticles was spherical according to transmission electron microscopy. In these studies, the antimicrobial studies of the AuNPS were exploited against different micro-organisms. The results of UV-VIS, FT-IR and TEM confirm that the leaves extract of Cannabis sativacan be used to produce gold nanoparticles with significant amount of antimicrobial activity.