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An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

Authors :
Corces, M Ryan
Trevino, Alexandro E
Hamilton, Emily G
Greenside, Peyton G
Sinnott-Armstrong, Nicholas A
Vesuna, Sam
Satpathy, Ansuman T
Rubin, Adam J
Montine, Kathleen S
Wu, Beijing
Kathiria, Arwa
Cho, Seung Woo
Mumbach, Maxwell R
Carter, Ava C
Kasowski, Maya
Orloff, Lisa A
Risca, Viviana I
Kundaje, Anshul
Khavari, Paul A
Montine, Thomas J
Greenleaf, William J
Chang, Howard Y
Source :
Nature Methods; October 2017, Vol. 14 Issue: 10 p959-962, 4p
Publication Year :
2017

Abstract

The Omni-ATAC protocol improves the signal-to-background ratio in chromatin accessibility profiles and is suitable for a range of cell lines and primary cell types, as well as frozen tissue.

Details

Language :
English
ISSN :
15487091 and 15487105
Volume :
14
Issue :
10
Database :
Supplemental Index
Journal :
Nature Methods
Publication Type :
Periodical
Accession number :
ejs43356588
Full Text :
https://doi.org/10.1038/nmeth.4396