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A chromosome conformation capture ordered sequence of the barley genome

Authors :
Mascher, Martin
Gundlach, Heidrun
Himmelbach, Axel
Beier, Sebastian
Twardziok, Sven O.
Wicker, Thomas
Radchuk, Volodymyr
Dockter, Christoph
Hedley, Pete E.
Russell, Joanne
Bayer, Micha
Ramsay, Luke
Liu, Hui
Haberer, Georg
Zhang, Xiao-Qi
Zhang, Qisen
Barrero, Roberto A.
Li, Lin
Taudien, Stefan
Groth, Marco
Felder, Marius
Hastie, Alex
Šimková, Hana
Staňková, Helena
Vrána, Jan
Chan, Saki
Muñoz-Amatriaín, María
Ounit, Rachid
Wanamaker, Steve
Bolser, Daniel
Colmsee, Christian
Schmutzer, Thomas
Aliyeva-Schnorr, Lala
Grasso, Stefano
Tanskanen, Jaakko
Chailyan, Anna
Sampath, Dharanya
Heavens, Darren
Clissold, Leah
Cao, Sujie
Chapman, Brett
Dai, Fei
Han, Yong
Li, Hua
Li, Xuan
Lin, Chongyun
McCooke, John K.
Tan, Cong
Wang, Penghao
Wang, Songbo
Yin, Shuya
Zhou, Gaofeng
Poland, Jesse A.
Bellgard, Matthew I.
Borisjuk, Ljudmilla
Houben, Andreas
Doležel, Jaroslav
Ayling, Sarah
Lonardi, Stefano
Kersey, Paul
Langridge, Peter
Muehlbauer, Gary J.
Clark, Matthew D.
Caccamo, Mario
Schulman, Alan H.
Mayer, Klaus F. X.
Platzer, Matthias
Close, Timothy J.
Scholz, Uwe
Hansson, Mats
Zhang, Guoping
Braumann, Ilka
Spannagl, Manuel
Li, Chengdao
Waugh, Robbie
Stein, Nils
Source :
Nature; April 2017, Vol. 544 Issue: 7651 p427-433, 7p
Publication Year :
2017

Abstract

Cereal grasses of the Triticeae tribe have been the major food source in temperate regions since the dawn of agriculture. Their large genomes are characterized by a high content of repetitive elements and large pericentromeric regions that are virtually devoid of meiotic recombination. Here we present a high-quality reference genome assembly for barley (Hordeum vulgare L.). We use chromosome conformation capture mapping to derive the linear order of sequences across the pericentromeric space and to investigate the spatial organization of chromatin in the nucleus at megabase resolution. The composition of genes and repetitive elements differs between distal and proximal regions. Gene family analyses reveal lineage-specific duplications of genes involved in the transport of nutrients to developing seeds and the mobilization of carbohydrates in grains. We demonstrate the importance of the barley reference sequence for breeding by inspecting the genomic partitioning of sequence variation in modern elite germplasm, highlighting regions vulnerable to genetic erosion.

Details

Language :
English
ISSN :
00280836 and 14764687
Volume :
544
Issue :
7651
Database :
Supplemental Index
Journal :
Nature
Publication Type :
Periodical
Accession number :
ejs41819311
Full Text :
https://doi.org/10.1038/nature22043