Molecular identification of trypanosome species in trypanotolerant cattle from the south of Gabon

The aim of this study was to provide information on trypanosome species infecting trypanotolerant cattle from southern Gabon. The study was conducted on 224 trypanotolerant cattle from three regions located in southern Gabon, using ITS1primer-based PCR. Seventy-two (32%) N’dama cattle were found polymerase chain reaction (PCR) positive with trypanosomes. The overall prevalence of trypanosomosis was 57% (63/110), 4% (4/100), and 36% (5/14) in the Gala section of the Nyanga ranch, the Miyama ranch, and Ossiele, respectively. Trypanosoma congolenseand Trypanosoma vivaxwere identified. In Gala section and Ossiele, T. congolenseand T. vivaxwere found. In the Miyama ranch, only T. vivaxwas identified. Mixed infections were also found. The forest(9%) and savannah(63%) subgroups of T. congolensewere identified. The presence of the two subgroups was detected in 16 out of 56 cattle (29%). T. congolenseand T. vivaxwould appear to be the main agents responsible for bovine trypanosomosis in southern Gabon. Although trypanotolerant, N’dama cattle may serve as a reservoir, and this should be further studied. On the other hand, these trypanotolerant cattle can be reared in such tsetse infested areas, which gives them an advantage compared to other trypanosensitive breeds, and this shows that they represent a key factor in biodiversity which has to be promoted.