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Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques

Authors :
D’Arco, Annalisa
Brancati, Nadia
Ferrara, Maria Antonietta
Indolfi, Maurizio
Frucci, Maria
Sirleto, Luigi
Source :
Biomedical Optics Express; May 2016, Vol. 7 Issue: 5 p1853-1864, 12p
Publication Year :
2016

Abstract

The visualization of heterogeneous morphology, segmentation and quantification of image features is a crucial point for nonlinear optics microscopy applications, spanning from imaging of living cells or tissues to biomedical diagnostic. In this paper, a methodology combining stimulated Raman scattering microscopy and image analysis technique is presented. The basic idea is to join the potential of vibrational contrast of stimulated Raman scattering and the strength of imaging analysis technique in order to delineate subcellular morphology with chemical specificity. Validation tests on label free imaging of polystyrene-beads and of adipocyte cells are reported and discussed.

Details

Language :
English
ISSN :
21567085
Volume :
7
Issue :
5
Database :
Supplemental Index
Journal :
Biomedical Optics Express
Publication Type :
Periodical
Accession number :
ejs38563270