Quantitative High-Pressure Liquid Chromatographic Determination of Thimerosal in Pharmaceutical Formulations

A quantitative high-pressure liquid chromatographic method using an anion-exchange resin column and an aqueous perchlorate solution as the mobile phase is employed for the determination of thimerosal in pharmaceutical formulations. With a liquid formulation containing large amounts of edetate disodium, calcium chloride is used for complexation to eliminate the interference from edetate disodium.