GLC Analysis of Trifluoperazine in Human Plasma

This report describes a sensitive gas chromatographic procedure for the measurement of trifluoperazine in human plasma. Trifluoperazine was extracted into heptane-2-propanol by a two-step-procedure and analyzed directly without derivatization. Prochlorperazine was employed as an internal standard because its structural and extraction characteristics were similar to trifluoperazine. The use of a nitrogen detection system reduced the number of interfering peaks. The within-day coefficient of variation in the method, over a 0.2–20-ng/ml concentration range was 9.4%.