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Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity
- Source :
- Cell Research; January 2016, Vol. 26 Issue: 1 p83-102, 20p
- Publication Year :
- 2016
-
Abstract
- Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron typing with the sensory phenotypes. Here we classify somatosensory neurons of the mouse dorsal root ganglion (DRG) by high-coverage single-cell RNA-sequencing (10 950 ± 1 218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors (MHNs). Each of the MHN types is further categorized into two subtypes. Large DRG neurons are categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors (MNs) expressing BAI1-associated protein 2-like 1 (Baiap2l1). Mechanoreceptors expressing trafficking protein particle complex 3-like and Baiap2l1-marked MNs are subdivided into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.
Details
- Language :
- English
- ISSN :
- 10010602 and 17487838
- Volume :
- 26
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Cell Research
- Publication Type :
- Periodical
- Accession number :
- ejs37615112
- Full Text :
- https://doi.org/10.1038/cr.2015.149