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A Technique for Locating Rare Cells in the Circulation for Ultramicroscopy1

Authors :
Gaynor, E.
Bouvier, C. A.
Cintron, J. R.
Source :
Experimental Biology and Medicine; February 1970, Vol. 133 Issue: 2 p520-523, 4p
Publication Year :
1970

Abstract

A method has been developed for the isolation of rare cells in heterogeneous cell suspensions, for ultramicroscopic examination. Such cells cannot be located using conventional embedding screening techniques. The cells are embedded in Epon which is polymerized in the shape of an ordinary glass slide. Large numbers of suspended cells may be screened by light microscopy for the rare cells, which may then be sectioned for ultramicroscopy. The method has been used to locate and ultramicroscopically identify circulating endothelial cells in the blood of patients and experimental animals.Although artifacts were induced by the techniques used for collecting and concentrating circulating endothelial cells, the embedding method itself did not cause further morphological alterations.

Details

Language :
English
ISSN :
15353702 and 15353699
Volume :
133
Issue :
2
Database :
Supplemental Index
Journal :
Experimental Biology and Medicine
Publication Type :
Periodical
Accession number :
ejs35571995
Full Text :
https://doi.org/10.3181/00379727-133-34510