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Alterations in Ecdysone Content during the Post-Embryonic Development of Chironomus thummi: Correlations with Chromosomal Puffing
- Source :
- Zeitschrift für Naturforschung C; August 1978, Vol. 33 Issue: 7-8 p557-560, 4p
- Publication Year :
- 1978
-
Abstract
- The ecdysone titer of larvae and pupae of Chironomus thummi was determined by radioimmune assay (RIA) and revealed a concentration of about 150 ng/g fresh weigth in late third instar larvae and a peak of more than 450 ng/g fresh weight just preceding pupation. The ecdysone titer curve shows a high degree of correlation with previously observed puffing activity at the ecdysone sensitive chromosomal site IIIdl. Further, β-ecdysone is the only endogenous ecdysone detected by high pressure liquid chromatography during larval-pupal development. It was also observed that developmentally arrested Chironomus contain less ecdysone than “normal” larvae.
Details
- Language :
- English
- ISSN :
- 09395075
- Volume :
- 33
- Issue :
- 7-8
- Database :
- Supplemental Index
- Journal :
- Zeitschrift für Naturforschung C
- Publication Type :
- Periodical
- Accession number :
- ejs35108856
- Full Text :
- https://doi.org/10.1515/znc-1978-7-818