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A high-throughput in vivomicronucleus assay for genome instability screening in mice

Authors :
Balmus, Gabriel
Karp, Natasha A
Ng, Bee Ling
Jackson, Stephen P
Adams, David J
McIntyre, Rebecca E
Source :
Nature Protocols; January 2015, Vol. 10 Issue: 1 p205-215, 11p
Publication Year :
2015

Abstract

This protocol describes a sensitive, robust, high-throughput method for quantifying the formation of micronuclei in mouse erythrocytes. It detects low levels of genome instability and can be used to identify novel genome maintenance genes and models.

Details

Language :
English
ISSN :
17542189 and 17502799
Volume :
10
Issue :
1
Database :
Supplemental Index
Journal :
Nature Protocols
Publication Type :
Periodical
Accession number :
ejs34580471
Full Text :
https://doi.org/10.1038/nprot.2015.010