Back to Search
Start Over
Carboxylation Reactions of Pyruvate: Ferredoxin Oxidoreductase and 2-Oxoglutarate: Ferredoxin Oxidoreductase from Hydrogenobacter thermophilusTK-6
- Source :
- Bioscience, Biotechnology, and Biochemistry; January 1997, Vol. 61 Issue: 3 p510-513, 4p
- Publication Year :
- 1997
-
Abstract
- Enzymatic reactions involving pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate: ferredoxin oxidoreductase from a thermophilic, aerobic, chemolithoautotrophic, and hydrogen-oxidizing bacterium, Hydrogenobacter thermophilusTK-6, were investigated as the CO2exchange reaction and CO2fixation reaction using ferredoxin isolated from the same organism as a reductant.The reduced ferredoxin was required in the pyruvate synthetic reaction and the 2-oxoglutarate synthetic reaction by a cell extract that had been treated with a PD-10 column to remove the low molecular weight substances. [14C]Pyruvate and [14C]2-oxoglutarate were detected as products of pyruvate synthetic and 2-oxoglutarate synthetic reactions, respectively. Further evidence for the operation of pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate: ferredoxin oxidoreductase was obtained from experiments on CO2exchange reactions using the purified enzymes.
Details
- Language :
- English
- ISSN :
- 09168451 and 13476947
- Volume :
- 61
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Publication Type :
- Periodical
- Accession number :
- ejs33078636
- Full Text :
- https://doi.org/10.1271/bbb.61.510