Basidiomycete Fungal Gene Encoding a Regulatory Subunit A Homologue of Protein Phosphatase 2A

A gene, Le.paa, encoding a regulatory subunit A (PR65) homologue of protein phosphatase 2A was isolated from the basidiomycete mushroom Lentinus edodes. The deduced Le.paagene product (Le.PR65) had the highest sequence similarity to the Schizosaccharomyces pombePR65 protein (54.1% similarity). The Le.paagene was shown to be transcribed more actively during the late stages of fruiting development of the fungus. Gill tissue in which basidiospores are formed contained abundant Le.paatranscript as compared with gill-depleted pileus and stipe.