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Characterization of Bacillus haloduransα-Galactosidase Mel4A Encoded by the mel4AGene (BH2228)
- Source :
- Bioscience, Biotechnology, and Biochemistry; September 2008, Vol. 72 Issue: 9 p2459-2462, 4p
- Publication Year :
- 2008
-
Abstract
- A family-4 α-galactosidase Mel4A of Bacillus haloduranswas expressed in Escherichia coliand characterized. Recombinant enzyme rMel4A depended on NAD+, some divalent cations such as Mn2+, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.
Details
- Language :
- English
- ISSN :
- 09168451 and 13476947
- Volume :
- 72
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Publication Type :
- Periodical
- Accession number :
- ejs32910821
- Full Text :
- https://doi.org/10.1271/bbb.80242