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The in situ expression of interleukin-8 in the normal human kidney and in different morphological forms of glomerulonephritis
- Source :
- American Journal of Kidney Diseases; June 2004, Vol. 43 Issue: 6 p983-998, 16p
- Publication Year :
- 2004
-
Abstract
- Background:Interleukin-8 (IL-8) is considered a deleterious chemokine involved in renal injury in glomerulonephritis (GN). IL-8 may be released as a 77-amino acid (AA) peptide or 72-AA protein. Methods:We evaluated gene and protein expression of IL-8 in 53 renal biopsy specimens from patients with GN and 9 control kidneys. Nonradioactive in situ hybridization and reverse-transcriptase polymerase chain reaction (RT-PCR) were applied to detect IL-8 messenger RNA (mRNA). In immunohistochemistry, a double-staining technique with the use of antibodies against the 77-AA and 72-AA forms of IL-8, as well as glomerular cell antigens, was used. Results:By in situ hybridization, IL-8 mRNA was detected in normal glomerular, tubular, and some interstitial cells. The RT-PCR study showed that IL-8 mRNA expression in control kidneys significantly exceeds that in specimens with GN (0.89 ± 0.82 versus 0.21 ± 0.20; P< 0.003). In control kidneys, major sources of 77-AA IL-8 were podocytes and endothelial cells of interstitial vessels, whereas tubular epithelial cells expressed minute amounts of 72-AA IL-8. In GN specimens, podocyte expression of 72-AA IL-8 varied notably, with the greatest level found in minimal change disease and the lowest level found in acute endocapillary GN. Conversely, increased glomerular expression of the 72-AA form of IL-8 was a general feature of GN, with its level significantly exceeding that of the 77-AA form in acute endocapillary GN (P< 0.01). Conclusion:Our results suggest that intrinsic glomerular cell production of IL-8, in particular the 77-AA form, may be relevant for preservation of the glomerular architecture.
Details
- Language :
- English
- ISSN :
- 02726386 and 15236838
- Volume :
- 43
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- American Journal of Kidney Diseases
- Publication Type :
- Periodical
- Accession number :
- ejs32612167
- Full Text :
- https://doi.org/10.1053/j.ajkd.2004.02.011