Oxidative Stress Induced by Copper and Iron Complexes with 8-Hydroxyquinoline Derivatives Causes Paraptotic Death of HeLa Cancer Cells

Here, we report the antiproliferative/cytotoxic properties of 8-hydroxyquinoline (8-HQ) derivatives on HeLa cells in the presence of transition metal ions (Cu2+, Fe3+, Co2+, Ni2+). Two series of ligands were tested, the arylvinylquinolinic L1–L8and the arylethylenequinolinic L9–L16, which can all interact with metal ions by virtue of the N,O donor set of 8-HQ; however, only L9–L16are flexible enough to bind the metal in a multidentate fashion, thus exploiting the additional donor functions. L1–L16were tested for their cytotoxicity on HeLa cancer cells, both in the absence and in the presence of copper. Among them, the symmetric L14exhibits the highest differential activity between the ligand alone (IC50= 23.7 μM) and its copper complex (IC50= 1.8 μM). This latter, besides causing a significant reduction of cell viability, is associated with a considerable accumulation of the metal inside the cells. Metal accumulation is also observed when the cells are incubated with L14complexed with other late transition metal ions (Fe3+, Co2+, Ni2+), although the biological response of HeLa cells is different. In fact, while Ni/L14and Co/L14exert a cytostatic effect, both Cu/L14and Fe/L14trigger a caspase-independent paraptotic process, which results from the induction of a severe oxidative stress and the unfolded protein response.