Simultaneous quantitation of naturally occurring insecticides, acaricides, and piscicides in rapeseed oil by UV-MALDI mass spectrometry

Quantitative UV-MALDI-Orbitrap mass spectrometric study of naturally occurring insecticides, acaricides and, piscicides rotenone (1), rotenolone (2), sumatrol (3), deguelin (4) as well as tephrosin (5) in rapeseed oil is presented. Direct emulsion assay and solid-state sample preparation techniques in presence of matrixes of organic crystals of ions are utilized. The protocol by APCI-MS and HPLC–ESI–MS/MS methods is verified. The method performance parameters along the matrix effects is evaluated by a comparison of analytical metrology achieved for rotenoids 1–5by each of mass spectrometric methods. Advantages of UV-MALDI-Orbitrap-MS method for quantitation are emphasized. They are compared to those of core analytical hybrid methods such as GC–MS, and HPLC–ESI (or APCI)-MS/MS for lipidomics and determination of organic pollutants in oils. The statistical simple random sampling plan is utilized for analysis of non-polluted rapeseed oils sample sets. The determination of polluted sample set and validation by spiked rapeseed oils is performed.