Involvement of MarR and YedS in Carbapenem Resistance in a Clinical Isolate of Escherichia colifrom China

ABSTRACTA carbapenem-resistant clinical isolate of Escherichia coli, which lacked OmpF and OmpC porins, carried a marRmutation and expressed a functional yedS, a normally nontranslated gene. MarR and YedS are described here as having effects on the ability of this strain to resist carbapenems. Additionally, expression of YedS was regulated by the small RNA MicF in a MarA-dependent way. These findings illustrate how broadly bacteria can mutate within a selective clinical setting, in this case, resistance to carbapenems, by altering three porin genes and one regulatory gene.