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TanA: a fluorogenic probe for thiaminase activityElectronic supplementary information (ESI) available: Experimental details. See DOI: 10.1039/c2ay26047
- Source :
- Analytical Methods; 2012, Vol. 5 Issue: 2 p446-448, 3p
- Publication Year :
- 2012
-
Abstract
- A fluorogenic thiamine analogue is presented as a fluorescent probe for thiaminase activity. The emission of the fluorophore is quenched by photoinduced electron transfer (PET) to the N-substituted pyridinium portion of the probe. Action of the enzyme releases the free pyridine group causing a substantial increase in fluorescence.
Details
- Language :
- English
- ISSN :
- 17599660 and 17599679
- Volume :
- 5
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Analytical Methods
- Publication Type :
- Periodical
- Accession number :
- ejs29211895
- Full Text :
- https://doi.org/10.1039/c2ay26047f