Systematic evaluation of medium-throughput mRNA abundance platforms

Although high-throughput techniques such as microarrays and RNA-seq are powerful methods for monitoring mRNA levels and alternative splicing, it remains important to validate a subset of the results using alternate technologies. In this paper, the authors perform a systematic evaluation of two medium-throughput platforms for measuring mRNA abundance. The results provide useful guidance to researchers considering alternative methods for validating high-throughput mRNA expression data.