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Soy Protein Isolate Reduces Hepatosteatosis in Yellow Avy/aMice Without Altering Coat Color Phenotype
- Source :
- Experimental Biology and Medicine; October 2008, Vol. 233 Issue: 10 p1242-1254, 13p
- Publication Year :
- 2008
-
Abstract
- Agouti (Avy/a) mice fed an AIN-93G diet containing the soy isoflavone genistein (GEN) prior to and during pregnancy were reported to shift coat color and body composition phenotypes from obese-yellow towards lean pseudoagouti, suggesting epigenetic programming. Human consumption of purified GEN is rare and soy protein is the primary source of GEN. Virgin a/a female and Avy/a male mice were fed AIN-93G diets made with casein (CAS) or soy protein isolate (SPI) (the same approximate GEN levels as in the above mentioned study) for 2 wks prior to mating. Avy/aoffspring were weaned to the same diets and studied at age 75 d. Coat color distribution did not differ among diets, but SPI-fed, obese Avy/aoffspring had lower hepatosteatosis (P< 0.05) and increased (P< 0.05) expression of CYP4a 14, a PPARα-regulated gene compared to CAS controls. Similarly, weanling male Sprague-Dawley (SD) rats fed SPI had elevated hepatic Acyl Co-A Oxidase (ACO) mRNA levels and increased in vitrobinding of PPARα to the PPRE promoter response element. In another hepatosteatosis model, adult SD rats fed a high fat/cholesterol diet, SPI reduced (P< 0.05) steatosis. Thus, 1) consumption of diets made with SPI partially protected against hepatosteatosis in yellow mice and in SD rats, and this may involve induction of PPARα-regulated genes; and 2) the lifetime (in utero, neonatal and adult) exposure to dietary soy protein did not result in a shift in coat color phenotype of Avy/amice. These findings, when compared with those of previously published studies of Avy/amice, lead us to conclude that: 1) the effects of purified GEN differ from those of SPI when GEN equivalents are closely matched; 2) SPI does not epigenetically regulate the agouti locus to shift the coat color phenotype in the same fashion as GEN alone; and 3) SPI may be beneficial in management of non-alcoholic fatty liver disease
Details
- Language :
- English
- ISSN :
- 15353702 and 15353699
- Volume :
- 233
- Issue :
- 10
- Database :
- Supplemental Index
- Journal :
- Experimental Biology and Medicine
- Publication Type :
- Periodical
- Accession number :
- ejs28907736
- Full Text :
- https://doi.org/10.3181/0802-RM-60