Separation of Polyhydroxylflavonoids by Packed-Column Supercritical Fluid Chromatography

Polyhydroxylflavonoids, isorhamnetin, kampcetin, quercetin, and fisetin are efficiently separated using supercritical carbon dioxide modified by ethanol with phosphoric acid as an additive on packed-column supercritical fluid chromatography. The effect of the silica-based stationary phase, composition of mobile phase, temperature, and pressure are investigated. The addition of phosphoric acid as a polar additive improves peak shapes.