Bioorthogonal Probes for Polo‐like Kinase 1 Imaging and Quantification

Click inside: A nuclear protein target, polo‐like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two‐step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins.