Production of SV-40 Complement-fixing Antigen

MAYER et al.1describe the preparation of complement-fixing antigen of vacuolating agent SV-40 in monkey kidney cell cultures. Strain No. 776 of SV -40 used for the work described here was obtained from Dr. H. M. Meyer. In our attempts to prepare a satisfactory complement-fixing antigen, two types of cell cultures were used: primary African green monkey kidney (AGMK) and serially propagated BS–C–l (ref. 1). These cultures were obtained from Microbiological Associates, Inc., and maintained at 34° C on Eagle's basal medium containing 2 per cent agamma ealf serum, 100 mg of streptomycin per ml., 100 units of penicillin per ml. and 2 mM of glutamine per ml.