Vanadium affects vacuolation and phosphate metabolism in Hansenula polymorpha

The yeast Hansenula polymorphais able to grow on vanadate concentrations that are toxic to other organisms. Transmission electron microscopy analysis showed that H. polymorphacells growing on a vanadate‐containing medium undergo a significant increase in cell vacuolation and a thickening of the cell wall; the presence of small cytoplasmic vesicles and an increase in cristae at the level of the plasma membrane were also observed. These ultrastructural modifications were accompanied by a change in the intracellular polyphosphate level, as shown by in vivo 31P‐NMR. The involvement of these observed changes in vanadium detoxification is discussed.